Spin-labeled hemoglobin.
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چکیده
منابع مشابه
Spin-labeled hemoglobin.
In the present paper we describe the results of a preliminary study of the paramagnetic resonance spectrum of spin-labeled horse hemoglobin in solution. Our results provide strong evidence that on oxygenation the A chains undergo a significant structural change near the "reactive" sulfhydryl group (f693), a change not yet resolved by X-ray diffraction.' We also find features in the paramagnetic...
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The paramagnetic resonance of a spin-label attached to a protein in solution, or in a single crystal, depends on static as well as on the dynamic features of its molecular environment.' This conformation-dependent paramagnetic resonance offers the possibility of relating structural properties of proteins in single crystals to those in solutions. The present paper is a brief report of a study of...
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A dog, doubly depleted of blood cells and plasma proteins, was fed dl-lysine labeled with C(14) in the epsilon carbon position. In the first 8 hours 28 per cent of the administered C(14) was excreted in the urine; in the first 72 hours, 35 per cent. Twenty-four hours after feeding, 4.2 per cent of the fed C(14) was circulating in the plasma, decreasing to 1 per cent at the end of 17 days. The C...
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In the next few minutes I shall present a very brief discussion of what the spin-label technique is, and how it may be used to study biological membranes, x, 2 A "spin label" is a synthetic organic paramagnetic free radical. Most spin
متن کاملSpin-labeled biomolecules.
are remarkably stable and inert,1 and show sharp, well-resolved, and simple paramagnetic resonance spectra2 that are sensitive to molecular motion,3 and, to a lesser extent, sensitive to polarity of the molecular environment.4 As shown below, such nitroxide radicals containing an isocyanate group can be bound to proteins and synthetic polypeptides in a manner similar to that used previously to ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1966
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.56.1.22